Banksy's Kissing Coppers is valued at $500,000-700,000 ahead of a street art auction at Fine Art Auctions Miami on February 18.
The painting first appeared outside the Prince Albert pub in Brighton, UK in 2004, and has since become one of the artist's most enduring images.
It will feature alongside work by Jean-Michel Basquiat, Keith Haring and Shepard Fairey.
In December last year, a Banksy mural achieved $204,000 at Julien's Auctions.
You can view our selection of art and photography memorabilia for sale here.
Our free newsletter keeps you up to date with all the latest on art and other collectibles.