Lady Gaga will consign her red Rolls-Royce Cormiche III to the May 17 Icons of Music sale at Julien's Auctions in Beverly Hills.
The lot is estimated to make around $30,000-50,000 for Musicares, a charity set up to provide healthcare and financial assistance for musicians.
The singer bought the car in 2009 and most notably used it as a prop in her perfume launch in 2012.
A spokesman for Julien's told the MailOnline: "Lady Gaga bought the car because she liked it and has owned it for quite some time, I believe around five years.
"She doesn't collect cars so this may well be the only car she has, so it is a pretty rare item. The title is in her real name, Stefani Germanotta, and it's the first time a car belonging to her is going up for sale.
"I think it will bring in interest from Lady Gaga fans and Rolls Royce fans, but it might be something a car museum would want to display."
We have this mask worn by Lady Gaga that was designed by the creator of her infamous meat dress.
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