A Gibson Ebony Dove guitar Elvis Presley played onscreen in his Aloha from Hawaii concert (1973) is to cross the block at Graceland Auctions.
It's expected to make $300,000-500,000 in the January 7 sale in Memphis.
The show was broadcast live from Honolulu around the world via satellite and attracted an estimated 1.5bn viewers - making it one of the biggest concerts of all time.
The auction house comments: "Graceland Auctions is pleased to present a varying array of amazing artifacts from Elvis' life and career.
"A plethora of unique and historical gems include the legendary guitar played by Elvis during the groundbreaking Aloha from Hawaii concert televised worldwide in 1973 and at over 100 additional live performances.
"Elvis' Gibson Ebony Dove is one of the most significant guitars ever to be offered publicly."
The lot could smash the current record for a piece of Elvis memorabilia, set at $300,000 for a 1953 acetate recording of My Happiness last year.
A letter Elvis wrote to his friend Alan "Hog Ears" Fortas while serving with the US military in Germany in 1958 will also feature. It's valued at $30,000-50,000.
He writes: "I can hardly wait to start singing, traveling, making movies, and above all seeing the old gang and old Graceland.
"All I do is sit and count the days. Well it'll be over in about 15 months and as Gen. MacArthur said, 'I shall return'."
Please sign up to our free newsletter to receive exciting news about music memorabilia auctions.