A 1966 Bizzarrini Strada will be auctioned by RM Sotheby's in London on September 7.
Giotto Bizzarrini was chief engineer at Ferrari during the 1950s and early 1960s.
He was behind some of the marque's most iconic designs, including the Ferrari 250 GTO - an example of which sold for a world record $52m in 2013.
He left in 1963 to form his own company.
RM Sotheby's comments: "This Bizzarrini GT Strada, chassis number 0248, was first titled in Italy in 1966, although the original owner is unknown.
"As a very early alloy example, it was actually built by Bizzarrini himself before Salvatore Diomante became his factory manager.
"It is believed that the car was raced in local hill climbs and rallies throughout Italy and France during the 1960s and '70s.
"The Bizzarini 5300 GT was created from the great mind that gave us the fabled Ferrari 250 GTO, and as such, it has equally aggressive looks and the race-bred performance of one of the most successful and highly prized of all 1960s sports cars."
There is no estimate available for the car, but a comparable specimen sold for $597,792 at RM Sotheby's in 2011.