The original prototype for NASA's Lunar Rover is to cross the block at RR Auction.
The lot is expected to sell for $125,000-150,000 in the online sale, which closes on April 21.
The Lunar Rover was first used on the Moon's surface during the Apollo 15 mission in 1971.
This first prototype was designed and built by Brown Engineering in 1965-1966 and was known as the Local Scientific Survey Module (LSSM).
This unique prototype was later sold to a scrapyard, where it remained for many years until it was rediscovered and authenticated last year.
Robert Livingston, RR Auction's executive vice-president, explained the appeal of space memorabilia to the Guardian newspaper: "The demographic tends to be men in their 50s who grew up watching men walk on the moon and are inspired by the achievement of the Apollo space program - so much of our scientific and computer use today is because of that.
"It was incredible - there's been nothing even close since.
"Now there's such a re-interest in launching space vehicles with the likes of Elon Musk, Richard Branson, Jeff Bezos - and they all grew up watching Apollo."
The sale will also include a Zeiss camera lens used on the Lunar surface during the Apollo 15 mission.
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