The 1958 Chevrolet Impala from George Lucas' hugely popular 1973 film American Graffiti will auction for $800,000-1.2m.
The lot will lead the third day of Profiles in History's Hollywood sale in Los Angeles on October 1.
In the film Steve Bolander (Ron Howard) lends the car to "The Toad" (Charles Martin Smith).
It's onscreen for much of the movie and is described by the auction house as being one of the most famous cars in American cinema history.
American Graffiti received huge critical acclaim on its release. It grossed an impressive $140m at the box office.
Profiles in History comments: "The Impala exemplified new possibilities. The car has been preserved and remained in the hands of its current private owner since 1973, purchased through an ad posted in The San Francisco Chronicle by George Lucas' Transportation Captain Henry Travers.
"The car features the original studio white paint with red trim, as well as the original studio-customized red and white tuck and roll upholstery from the film and original wheels…
"This is the first time in 42 years the car is available for sale to the public."
Please sign up to our free newsletter to receive exciting news about movie memorabilia auctions.