Banksy's Happy Choppers (2002) will star in a sale of street and contemporary art at Julien's Auctions.
The lot is expected to sell for $100,000-150,000 when it crosses the block in LA on April 30.
It's one of a number of pieces of his work offered in the sale.
The auction house comments: "This board originally was used to prevent the public from accessing a construction site in Central London at the Whitecross Street Market in 2002.
"This street work is an early example of Banksy's helicopter motif, an image which he has revisited numerous times and has since become an iconic part of his repertoire."
The piece was last owned by Banksy's former manager Steve Lazarides.
Heart Boy (2009), another work by Banksy, is also up for grabs - with an estimate of $80,000-120,000.
The work was a collaboration with Brooklyn graffiti collective Faile and was recovered from a building in Islington in 2009 just prior to demolition.
The stencil can be seen in a variety of other locations across London.
Banksy's record remains $1.8m, set for Keep it Spotless (a collaboration with Damien Hirst) at Sotheby's in 2008.
The sale will include work from a wealth of other artists, including Shepard Fairey, Andy Warhol and Keith Haring.
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